Lyophilized EGFP Fluorescent EVs from HEK293 cells

FluoEVs are fluorescently labeled extracellular vesicles designed for imaging and tracking applications. Unlike regular EVs, FluoEVs contain stable fluorescent markers that allow researchers to visualize EV uptake, biodistribution, and cellular interactions in real-time. Our proprietary labeling technology ensures the fluorescence remains stable for up to 7 days without compromising EV integrity or biological function.

Proper storage is crucial for maintaining EV integrity and functionality:

• • Store at -80°C for long-term storage (up to 12 months)
• • Short-term storage (up to 1 week) at 4°C is acceptable
• • Avoid repeated freeze-thaw cycles
• • Always thaw on ice and mix gently before use

All our EV products undergo comprehensive quality control testing:

• • Nanoparticle Tracking Analysis (NTA) for size distribution and concentration
• • Flow cytometry for surface marker characterization
• • Sterility testing (USP <71> compliant)
• • Endotoxin testing (<0.5 EU/mL)
• • Mycoplasma testing by PCR

The choice of isolation method depends on your specific requirements:

The optimal EV concentration varies depending on the application and cell type. As a starting point, we recommend:

• For uptake studies: 10-50 µg/mL (protein concentration) or 1×10⁸-1×10⁹ particles/mL
• For functional studies: 50-200 µg/mL or 1×10⁹-1×10¹⁰ particles/mL
• For in vivo studies: 1×10¹⁰-1×10¹¹ particles per injection

We recommend performing dose-response experiments to determine the optimal concentration for your specific application.

All EV products are shipped on dry ice to maintain product integrity. We use specialized packaging to ensure temperature stability during transit. Express shipping is standard to minimize transit time. Upon receipt, products should be immediately stored at -80°C or used according to the product instructions. We provide tracking information and monitor all shipments to ensure safe delivery.

Yes, we offer custom packaging options for most of our products. We can provide:

• Custom volumes (from 100 µL to 10 mL)
• Specific particle concentrations
• Multiple aliquots to avoid freeze-thaw cycles
• Custom buffer formulations

Please contact our sales team with your specific requirements for a custom quote. Minimum order quantities may apply for custom preparations.

We can process a wide variety of biological samples for EV isolation:

• Blood products: Plasma, serum, platelet concentrates
• Cell culture: Conditioned media from any cell type
• Body fluids: Urine, saliva, CSF, synovial fluid
• Tissue samples: With specialized processing protocols

Each sample type requires specific pre-processing steps. Our technical team will work with you to develop the optimal protocol for your samples.

Turnaround times vary depending on the service complexity:

• Standard EV isolation: 2-3 weeks
• Characterization services: 3-4 weeks
• Omics analysis: 4-6 weeks
• Custom protocol development: 6-8 weeks

Rush services are available for certain applications. All projects include regular updates and a comprehensive final report with your data.